VRE Broth
Enrichment medium for detection of Vancomycin-Resistant Enterococci (VRE)
Vancomycin-resistant enterococci (VRE) represent a serious challenge in clinical microbiology and infection control. Resistance is mediated by the van genes (vanA, vanB, vanC), which can be plasmid-borne or chromosomally encoded. The rapid identification of VRE carriers is crucial to prevent spread in healthcare facilities.
Method Principle
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Inoculate the broth with the sample.
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Incubate at 35 ± 2 °C for 24–48 h.
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Positive result: Turbidity and blackening of the medium.
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Subculture for complete identification.
VRE Broth (Liofilchem®) is a selective enrichment medium designed for the screening, isolation, and cultivation of vancomycin-resistant enterococci.
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Contains vancomycin to inhibit susceptible organisms.
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Selective components (aesculin, bile, azide) support the growth and differentiation of enterococci.
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Aesculin hydrolysis produces a black color reaction in positive cases.
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Sensitive: Detects VRE carriers with low bacterial concentrations
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Fast: Growth and color change visible after 24–48 h incubation at 35 °C
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Simple: Ready-to-use tubes, easy to handle in routine labs
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Specific: Inhibits Gram-negative bacteria and vancomycin-susceptible enterococci